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詳細(xì)解析Biolegend流式細(xì)胞的工作原理
發(fā)布時(shí)間： 2022-02-21 點(diǎn)擊次數(shù)： 1181次Biolegend流式細(xì)胞技術(shù)是利用流式細(xì)胞儀進(jìn)行的一種單細(xì)胞定量分析和分選技術(shù)。流式細(xì)胞術(shù)是單克隆抗體及免疫細(xì)胞化學(xué)技術(shù)、激光和電子計(jì)算機(jī)科學(xué)等高度發(fā)展及綜合利用的高技術(shù)產(chǎn)物。它是在細(xì)胞分子水平上通過單克隆抗體對單個(gè)細(xì)胞或其他生物粒子進(jìn)行多參數(shù)、快速的定量分析。它可以高速分析上萬個(gè)細(xì)胞，并能同時(shí)從一個(gè)細(xì)胞中測得多個(gè)參數(shù)，具有速度快、精度高、準(zhǔn)確性好的優(yōu)點(diǎn)，是當(dāng)代*的細(xì)胞定量分析技術(shù)之一。基本結(jié)構(gòu)：Biolegend流式細(xì)胞主要是由三部分構(gòu)成：1、液流系統(tǒng)，包括流動室和液流驅(qū)動系統(tǒng)；2、光學(xué)系統(tǒng)，包括激發(fā)光源和光束收集系統(tǒng)；3、電子系統(tǒng)，包括光電轉(zhuǎn)換器和數(shù)據(jù)處理系統(tǒng)。Biolegend流式細(xì)胞儀的工作原理：是使懸浮在液體中分散的經(jīng)熒光標(biāo)記的細(xì)胞或微粒逐個(gè)通過樣品池，同時(shí)由熒光探測器捕獲熒光信號并轉(zhuǎn)換成分別代表前向散射角、側(cè)向散射角和不同熒光強(qiáng)度的電脈沖信號，經(jīng)計(jì)算機(jī)處理形成相應(yīng)的點(diǎn)圖，直方圖和加三維結(jié)構(gòu)圖像進(jìn)行分析。用于FCM的樣本是單細(xì)胞懸液，可以是血液、懸浮細(xì)胞培養(yǎng)液、各種體液、新鮮實(shí)體瘤的單細(xì)胞懸液以及石蠟包埋組織的單細(xì)胞懸液等。
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